The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Sep. 04, 2018
Filed:
Feb. 19, 2009
Benjamin Fryer, Skillman, NJ (US);
Shelley Nelson, Skillman, NJ (US);
Villy Nielsen, Roskilde, DK;
Tina Kristensen Marwood, Hvalsø, DK;
Thomas Brevig, Jyllinge, DK;
Benjamin Fryer, Skillman, NJ (US);
Shelley Nelson, Skillman, NJ (US);
Villy Nielsen, Roskilde, DK;
Tina Kristensen Marwood, Hvalsø, DK;
Thomas Brevig, Jyllinge, DK;
Janssen Biotech Inc., Horsham, PA (US);
Nunc A/S, Horsham, PA (US);
Abstract
The present invention relates to the field of mammalian cell culture, and provides methods and compositions for cell attachment to, cultivation on and detachment from a solid substrate surface containing from at least about 0.5% N, a sum of O and N of greater than or equal to 17.2% and a contact angle of at least about 13.9 degrees, lacking a feeder cell layer and lacking an adlayer. In one embodiment of the present invention, the cells are treated with a compound capable of inhibiting Rho kinase activity. In another embodiment, the cells are treated with a compound capable of inhibiting Rho activity.