Si-Hyoung Lee

Title: Si-Hyoung Lee: Inventor in Recombinant DNA Library Methods

Introduction

Si-Hyoung Lee is an inventor associated with Jinju, South Korea, and Amicogen, Inc. Lee’s work includes a patent on a method for generating a recombinant DNA library using unidirectional single-stranded DNA fragments. The supplied information lists 1 patent.

Latest Patents

Lee’s patent, “Method for Generating Recombinant DNA Library Using Unidirectional Single-Stranded DNA Fragments,” describes producing recombinant polynucleotides from two or more homologous double-stranded polynucleotides. The method generates unidirectional single-stranded fragments, uses them as templates in repeated extension reactions with specific oligonucleotide primers, and amplifies the resulting recombinant polynucleotides through polymerase chain reaction.

The patent also describes constructing a recombinant DNA library by inserting the recombinant polynucleotides into a vector and introducing that vector into expression cells to produce mutant clones. The approach is intended to support in vitro recombination of homologous polynucleotides and directed molecular evolution.

Career Highlights

Lee is associated with Amicogen, Inc., a company connected to biotechnology innovation. The patent’s focus on recombination and library construction reflects an application of molecular biology techniques to generate and evaluate genetic variants.

Collaborations

The supplied information identifies Eun-Jung Ryu and Yeong-Joong Jeon as Lee’s coworkers. No further details about their roles or specific contributions to the patent are provided.

Conclusion

Si-Hyoung Lee’s listed patent presents a method for creating recombinant polynucleotides and mutant-clone libraries, with potential use in directed molecular evolution.