The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Oct. 18, 2005
Filed:
Jun. 16, 2001
Si-hyoung Lee, Jinju-si, KR;
Yong-chul Shin, Jinju-si, KR;
Yeong-joong Jeon, Seoul, KR;
Kyung-hwa Jung, Daejeon, KR;
Eun-jung Ryu, Jinju-si, KR;
Ko-woon Lee, Jinju-si, KR;
Si-Hyoung Lee, Jinju-si, KR;
Yong-Chul Shin, Jinju-si, KR;
Yeong-Joong Jeon, Seoul, KR;
Kyung-Hwa Jung, Daejeon, KR;
Eun-Jung Ryu, Jinju-si, KR;
Ko-Woon Lee, Jinju-si, KR;
Amicogen, Inc., , KR;
Abstract
The present invention relates to a method for producing a recombinant polynucleotides comprising the steps of generating a pool of unidirectional single-stranded polynucleotide fragments from two or more homologous double-stranded polynucleotides, conducting a polymerization process comprising multi-cyclic extension reactions using the unidirectional single-stranded polynucleotide fragments as templates and specific oligonucleotides as primers to obtain recombinant polynucleotides, and conducting a polymerase chain reaction using at least one primer to amplify the recombinant polynucleotides; and a method for constructing a recombinant DNA library comprising the steps of inserting the recombinant polynucleotide prepared by the above method into a vector and transforming an expression cell with said vector containing the recombinant polynucleotide to obtain a plurality of mutant clones. The method of the present invention can be used for in vitro recombination of homologous polynucleotides and the directed molecular evolution.