Richard St John

Title: Richard St John: Innovator in Protein Refolding Technologies

Introduction

Richard St John is a notable inventor based in San Francisco, CA (US). He has made significant contributions to the field of protein refolding, holding a total of 6 patents. His work focuses on developing methods that enhance the recovery of biologically active proteins from denatured states.

Latest Patents

One of his latest patents, titled "High pressure refolding of protein aggregates and inclusion bodies," presents an effective method for refolding denatured proteins in solution. This innovative approach allows for the recovery of properly folded proteins in high yield by applying pressures between about 0.25 kbar to about 3.5 kbar, with optimal results typically achieved at around 1.5 kbar to 3 kbar. The method incorporates a chaotropic agent at a concentration that does not denature proteins at atmospheric pressure, and it may also include oxidation-reduction reagents to facilitate the formation of native intramolecular disulfide bonds. This technique is applicable to a wide range of proteins, particularly after the solubilization or denaturation of insoluble protein aggregates and inclusion bodies.

Another significant patent by St John focuses on "Methods for protein refolding." This invention outlines improved techniques for disaggregating protein aggregates and refolding denatured proteins using high pressure. The methods described involve agitation, high temperature, 'stepped' depressurization, dialysis, and dilution under pressure to enhance the speed and extent of aggregate dissolution and protein refolding.

Career Highlights

Throughout his career, Richard St John has worked with various organizations, including Barofold, Inc. and University Technology Corporation. His innovative approaches have contributed to advancements in biochemistry and biotechnology.

Collaborations

St John has collaborated with notable individuals in his field, including Theodore W Randolph and John Frank Carpenter. These partnerships have further enriched his research and development efforts.

Conclusion

Richard St John is a prominent figure in the realm of protein refolding technologies, with a focus on high-pressure methods that enhance the recovery of biologically active proteins. His contributions continue to influence the field and pave the way for future innovations.