Qian Li

Title: Qian Li: Innovator in Nitrilase Mutants

Introduction

Qian Li is a prominent inventor based in Zhejiang, China. He has made significant contributions to the field of biochemistry, particularly in the development of nitrilase mutants. His work focuses on enhancing enzyme activity for industrial applications.

Latest Patents

Qian Li holds a patent for a nitrilase mutant and its application in the synthesis of 1-cyanocyclohexyl acetic acid. The invention details a nitrilase mutant obtained by mutating one or two amino acids at positions 180 and 205 of the amino acid sequence shown in SEQ ID No. 2. Through semi-rational design and protein molecular modification, the specific enzyme activity of the nitrilase double mutant AcN-G180D/A205C was increased by up to 1.6 folds, achieving a conversion rate greater than 99%. The reaction time was also significantly reduced to a quarter of the original duration when using the recombinant containing the nitrilase mutant to hydrolyze 1-cyanocyclohexylacetonitrile at high temperatures of 50° C. This innovation holds promising potential for efficiently catalyzing the synthesis of gabapentin intermediate, 1-cyanocyclohexyl acetic acid.

Career Highlights

Qian Li is affiliated with Zhejiang University, where he conducts his research and development activities. His work has garnered attention for its practical applications in the pharmaceutical industry, particularly in the synthesis of important chemical intermediates.

Collaborations

Qian Li has collaborated with notable colleagues, including Yaping Xue and Neng Xiong, to advance his research in enzyme technology and its applications.

Conclusion

Qian Li's innovative work in the field of nitrilase mutants exemplifies the potential of biochemistry in industrial applications. His contributions are paving the way for more efficient synthesis processes in the pharmaceutical sector.