Martha B Ladner

Title: Martha B. Ladner: Innovator in AAV Production and Immune Regulation

Introduction

Martha B. Ladner is a prominent inventor based in Oakland, California, known for her significant contributions to the field of biotechnology. With a total of 14 patents to her name, she has made remarkable advancements in the production of adeno-associated viruses (AAV) and the regulation of the immune system.

Latest Patents

One of her latest patents focuses on "Materials and methods for simplified AAV production." This invention provides a method for producing purified replication-defective recombinant AAV virions. The method involves introducing an AAV vector, an AAV helper construct, and an adenoplasmid accessory construct into a suitable host cell. The adenoplasmid accessory plasmid is designed to be unable to be packaged into adenoviral particles due to the absence of packaging signal sequences or because it is too large to package. The host cell is then cultured to produce crude rAAV virions, which are subsequently lysed. The resulting cell lysate is processed through a chromatographic column containing sulfonated cellulose or subjected to cesium chloride equilibrium gradient centrifugation to recover the purified rAAV virions.

Another significant patent involves "DNA encoding N∇2 CSF-1 (short form)." This invention relates to a colony-stimulating factor, CSF-1, which is a lymphokine useful in regulating the immune system. It is particularly beneficial in overcoming immunosuppression induced by chemotherapy or other causes. CSF-1 can be obtained in usable amounts through recombinant methods, including the cloning and expression of murine and human DNA sequences encoding this protein. Both "long" and "short" forms of this protein, along with muteins corresponding to the cDNA-encoded forms, are disclosed in this patent.

Career Highlights

Martha has worked with notable companies such as Chiron Corporation and Cetus Corporation, where she has applied her expertise in biotechnology and innovation. Her work has significantly impacted the development of therapeutic solutions in the medical field.

Collaborations

Throughout her career, Martha has collaborated with esteemed colleagues, including Ernest S. Kawasaki and Mazie Yee Coyne. These collaborations have further enhanced her contributions to the field and have fostered advancements in biotechnology.

Conclusion

Martha B. Ladner is a