Location History:
- SV Rijen, NL (1993)
- Rijen, NL (1993 - 2000)
Company Filing History:
5 out of 236 
1 out of 64 
1 out of 475 
Years Active: 1993-2000
Title: Innovations of Leo J Mulleners
Introduction
Leo J Mulleners is a notable inventor based in Rijen, Netherlands. He has made significant contributions to the field of biotechnology, particularly in the area of gene amplification and the production of polypeptides. With a total of seven patents to his name, Mulleners has developed innovative methods that enhance the capabilities of prokaryotic hosts.
Latest Patents
Mulleners' latest patents focus on stable gene amplification in chromosomal DNA of prokaryotic organisms. His work involves transformed prokaryotic hosts that maintain multiple copies of a DNA sequence within their chromosomes. This DNA sequence encodes a polypeptide of interest, with the copies separated by endogenous chromosomal DNA sequences. The methods he developed allow for the production of transformed host strains that can produce increased amounts of the desired polypeptide compared to existing strains. Preferred host strains include Bacillus novo species PB92, known for its high-alkaline proteolytic enzyme production, and B. licheniformis strain T5, which produces a thermostable alpha-amylase. Mulleners has also introduced novel methods for creating industrial Bacillus strains that enhance the production of both endogenous and exogenous polypeptides.
Career Highlights
Throughout his career, Mulleners has worked with prominent companies in the biotechnology sector, including Gist-Brocades N.V. and Gist-Brocades B.V. His expertise in genetic engineering and microbial production has positioned him as a key figure in the development of innovative biotechnological solutions.
Collaborations
Mulleners has collaborated with notable colleagues such as Johan Pieter Sanders and Peter Michael Andreoli. These partnerships have contributed to the advancement of his research and the successful implementation of his patented technologies.
Conclusion
Leo J Mulleners is a distinguished inventor whose work in gene amplification and microbial production has made a significant impact in biotechnology. His innovative patents and collaborations highlight his contributions to the field, showcasing the importance of his research in enhancing polypeptide production.