The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jun. 08, 1993

Filed:

Feb. 29, 1988
Applicant:
Inventors:

Christiaan A van Eekelen, Bergschenhoek, NL;

Johannes C van der Laan, Amsterdam, NL;

Leo J Mulleners, Rijen, NL;

Assignee:
Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
C12P / ; C12N / ; B12N / ;
U.S. Cl.
CPC ...
435 691 ; 4351723 ; 435221 ; 43525231 ; 4353201 ;
Abstract

Novel methods and novel industrial Bacillus strains are provided for enhanced production of serine protease. Plasmid constructs comprising the serine protease gene are introduced into a compatible host, generally a Bacillus already overproducing serine protease. Preferred host cells are those from Bacillus novo sp. PB92 and the preferred serine protease gene also originates from Bacillus PB92. Integration and maintenance of the plasmid in the host cell chromosome can be achieved by including a temperature sensitive origin of replication in the plasmid construct and growing under selective conditions.


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