Laura L Stuve

Title: Laura L Stuve: Innovator in cDNA Library Construction

Introduction

Laura L Stuve is a prominent inventor based in Los Gatos, CA. She has made significant contributions to the field of molecular biology, particularly in the construction of cDNA libraries. With a total of 7 patents to her name, her work has advanced the methods used in DNA sequencing and cDNA synthesis.

Latest Patents

One of her latest patents focuses on the "Construction of uni-directionally cloned cDNA libraries from messenger RNA for improved 3' end DNA sequencing." This invention provides methods for preparing cDNA corresponding to mRNA. In her methods, mRNA is first contacted with a mixture of primers under first strand cDNA synthesis conditions. The primer mixture contains primers with at least 10 contiguous deoxythymidines, a double-stranded restriction enzyme recognition sequence near one end, and a non-polyA-complementary region near the other end. The resultant cDNA is modified to substantially remove the polyT tail and is then ligated into a vector. These methods are particularly useful in DNA sequencing and the synthesis of cDNA libraries.

Career Highlights

Laura has worked with notable companies such as Incyte Pharmaceuticals, Inc. and Incyte Genomics, Inc. Her experience in these organizations has allowed her to refine her skills and contribute to groundbreaking research in her field.

Collaborations

Throughout her career, Laura has collaborated with esteemed colleagues, including Glenn K Fu and Philippe E Rigault. These partnerships have further enriched her work and expanded the impact of her inventions.

Conclusion

Laura L Stuve is a remarkable inventor whose contributions to cDNA library construction have significantly advanced molecular biology. Her innovative methods and collaborations continue to influence the field, showcasing her dedication to scientific progress.