Incyte Pharmaceuticals, Inc.

Incyte Genomics, Inc.

Incyte Corporation

Laura L Stuve

Glenn K Fu

Michael Graham Walker

Walter H Lee

Tod M Klingler

Richard D Goold

Harold H Hibbert

Michael P Wood

Robert E Lagace

Steven Starnes

Anne L Curtis

Philippe E Rigault

David A Hadley

Susan G Stuart

James Randall Gilmore

Steven Daniel

Irene Ni

Jonathan T Wang

Methods are provide for preparing cDNA corresponding to a mRNA. In the subject methods, a mRNA is first contacted with a mixture of primers under first strand cDNA synthesis conditions. The primer mixture contains primers that have at least 10 contiguous deoxythymidines, a double stranded restriction enzyme recognition sequence near one end and a non-polyA-complementary region near the other end, where the non-polyA-complementary region is -VV, -VTV, -VTTV, -VTTTV, and -VVVVV. The resultant cDNA is modified such that the polyT tail is substantially removed. The modified cDNA is then ligated into a vector. The subject methods find use in a variety of applications, and find particular use in the sequencing of DNA and in the synthesis of cDNA libraries.

Construction of uni-directionally cloned cDNA libraries from messenger RNA for improved 3' end DNA sequencing

The present invention provides new methods of synthesizing cDNAs, methods of verifying full-length cDNAs, methods of producing cDNA libraries enriched for full-length inserts, and the like.

cDNA libraries and methods for their production

Construction of uni-directionally cloned cDNA libraries from messenger RNA for improved 3&prime; end DNA sequencing

A computer system stores biomolecular data in a database in a memory. The biomolecular database has a set of entities. Each entity stores attributes for a plurality of entries. At least one attribute is stored in an array. Data associated with an entry is stored at a location in the array. An entity offset designates the location of the data in the array. The same entity offset value is used to access data associated with a particular entry for all attributes within the entity.

System and method for a precompiled database for biomolecular sequence information

The present invention relates to polynucleotides comprising genes that regulate cell proliferation. The present invention also relates to a method for diagnosing or monitoring the treatment of a disease characterized by the altered expression of genes that regulate cell proliferation in a sample.

Detection of altered expression of genes regulating cell proliferation

The present invention provides methods for determining nucleic acid sequences based on known sequences adjacent to the unknown sequences, and reagents for using such methods.

Method for isolating and characterizing nucleic acid sequences

Growing community of inventors

Los Gatos, CA, United States of America

Laura L Stuve