Location History:
- Taipei, TW (2013 - 2014)
- Changhwa County, TW (2019)
Company Filing History:
Years Active: 2013-2019
Title: Kuo Ping Chiu: Innovator in Nucleic Acid Amplification
Introduction
Kuo Ping Chiu is a prominent inventor based in Taipei, Taiwan. He has made significant contributions to the field of molecular biology, particularly in the amplification of nucleic acids. With a total of 3 patents, his work has advanced the methodologies used in genetic research and diagnostics.
Latest Patents
Kuo Ping Chiu's latest patents include innovative methods for full-length amplification of double-stranded linear nucleic acids of unknown sequences. This invention discloses an adaptor that facilitates the amplification of all linear, double-stranded nucleic acid molecules in a sample. The adaptor consists of two oligonucleotides: the first oligonucleotide (P-oligo) has a phosphate at the 5' end and lacks an additional thymine nucleotide at the 3' end, while the second oligonucleotide (T-oligo) includes an extra 3'-T and does not have a 5'-phosphate. These oligonucleotides are complementary except at the 3'-T in the T-oligo. The adaptor is ligated to nucleic acids with an extra A in the 3' end, forming adaptor-ligated target nucleic acids. The T-oligo is then used as a single primer for T-oligo-primed polymerase chain reaction (TOP-PCR), enabling the full-length amplification of unknown nucleic acids.
Another notable patent involves a multiplex barcoded paired-end diTag (mbPED) sequencing approach. This method generates a barcoded Paired-End Ditag (bPED) nucleic acid fragment through a series of ligation and digestion steps. The process includes ligating a half-adaptor to a target nucleic acid, circularizing the construct, and digesting it to produce the bPED fragment. This innovative approach has applications in fusion gene identification.
Career Highlights
Kuo Ping Chiu is affiliated with Academia Sinica, a leading research institution in Taiwan. His work has been instrumental in enhancing the techniques used in genetic research, making significant strides in the understanding of nucleic acids.
Collaborations
He has collaborated with notable colleagues, including Yu-Shin Nai and Yi Hung Chou, contributing to various research projects and advancements in molecular biology.
Conclusion