Keisuke Kurosaka

Title: Keisuke Kurosaka: Innovator in Protein Purification and Glucose Dehydrogenase Production

Introduction

Keisuke Kurosaka is a notable inventor based in Uji, Japan. He has made significant contributions to the field of biochemistry, particularly in the purification of proteins and the production of glucose dehydrogenases. With a total of 5 patents to his name, his work has implications for various applications in biotechnology.

Latest Patents

Kurosaka's latest patents include a method for purifying proteins and a process for producing glucose dehydrogenases. The method for purifying proteins involves liquid chromatography, where a protein solution containing the target protein is introduced into a column filled with a packing agent. This process allows for the bonding of the packing agent to the target protein, followed by the removal of impurities and elution of the target protein using an eluent containing hydroxy-cholate. One example of the target protein is glucose dehydrogenase, which has glucose dehydrogenation activity. The chromatography is performed as a combination of hydrophobic chromatography and anion exchange chromatography.

The process for producing glucose dehydrogenases involves transferring a DNA sequence that encodes an α subunit with glucose dehydrogenase activity and a β subunit that acts as an electron transfer protein into a microorganism. This results in the construction of a transformant that is cultured to produce glucose dehydrogenases. The α subunit has a molecular weight of approximately 60 kDa, while the β subunit weighs about 43 kDa.

Career Highlights

Throughout his career, Kurosaka has worked with prominent companies such as Unitika Ltd. and Arkray, Inc. His experience in these organizations has contributed to his expertise in biochemistry and innovation in protein purification techniques.

Collaborations

Kurosaka has collaborated with notable coworkers, including Shido Kawase and Hideaki Yamaoka. These partnerships have likely enhanced his research and development efforts in the field.

Conclusion

Keisuke Kurosaka is a distinguished inventor whose work in protein purification and glucose dehydrogenase production has made a significant impact in biotechnology. His innovative methods and collaborative efforts continue to advance the field and inspire future research.