The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
May. 11, 2010

Filed:

Aug. 20, 2003
Applicants:

Koji Sode, Tokyo, JP;

Hideaki Yamaoka, Kyoto, JP;

Mitsuhiro Hoshijima, Kyoto, JP;

Keisuke Kurosaka, Kyoto, JP;

Shido Kawase, Kyoto, JP;

Inventors:

Koji Sode, Tokyo, JP;

Hideaki Yamaoka, Kyoto, JP;

Mitsuhiro Hoshijima, Kyoto, JP;

Keisuke Kurosaka, Kyoto, JP;

Shido Kawase, Kyoto, JP;

Assignee:

ARKRAY, Inc., Kyoto, JP;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 1/21 (2006.01); C07H 21/04 (2006.01);
U.S. Cl.
CPC ...
Abstract

A process for producing glucose dehydrogenases. This process comprises transferring a DNA containing a sequence represented by SEQ ID NO:1 which encodes an α subunit having a glucose dehydrogenase activity and a β subunit being an electron transfer protein into a microorganism belonging to the genusto thereby construct a transformant, and culturing this transformant so as to allow the production of a first glucose dehydrogenase containing the above-described β subunit and a second glucose dehydrogenase free from the β subunit. The α subunit as described above has a molecular weight of about 60 kDa measured by, for example, SDS-polyacrylamide gel electrophoresis under reducing conditions, while the β subunit as described above has a molecular weight of about 43 kDa measured by, for example, SDS-polyacrylamide gel electrophoresis under reducing conditions.


Find Patent Forward Citations

Loading…