Keishin Sugawara

Title: Keishin Sugawara: Innovator in Viral Propagation and Protein Purification

Introduction

Keishin Sugawara is a notable inventor based in Kumamoto, Japan. He has made significant contributions to the fields of virology and biochemistry, holding three patents that showcase his innovative approaches to scientific challenges.

Latest Patents

One of Sugawara's latest patents is a method for propagating influenza virus. This method provides a way to produce influenza virus on a large scale by removing or decreasing a trypsin inhibitor secreted into the culture of MDCK cells. The process involves washing the cells with a culture medium or buffer, inoculating them with the influenza virus, and culturing the cells in a medium supplemented with trypsin. Another significant patent is a method of purifying calcium ion-binding protein. This invention relates to a simple and efficient method for isolating and purifying calcium ion-binding proteins from liquid samples. The method utilizes cation exchange chromatography in the presence of calcium ions, allowing for the adsorption of the protein to the carrier, followed by washing and elution to obtain a purified product.

Career Highlights

Throughout his career, Sugawara has worked with esteemed organizations such as the Chemo-Sero-Therapeutic Research Institute and Teijin Limited. His work in these institutions has allowed him to develop and refine his innovative methods, contributing to advancements in medical and biochemical research.

Collaborations

Sugawara has collaborated with notable colleagues, including Toshinobu Nouchi and Kiyoto Nishiyama. These partnerships have fostered a collaborative environment that enhances the development of innovative solutions in their respective fields.

Conclusion

Keishin Sugawara's contributions to the fields of virology and biochemistry through his patents demonstrate his commitment to innovation and scientific advancement. His work continues to impact the scientific community positively.