The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Mar. 22, 2005
Filed:
Jul. 18, 2001
Hiroshi Mizokami, Kumamoto, JP;
Shinichi Furukawa, Kumamoto, JP;
Keishin Sugawara, Kumamoto, JP;
Tatsufumi Onchi, Kumamoto, JP;
Kazuhiro Komatsu, Kumamoto, JP;
Satoshi Koyanagi, Kumamoto, JP;
Hideo Yoshizaki, Sayama, JP;
Hiroshi Mizokami, Kumamoto, JP;
Shinichi Furukawa, Kumamoto, JP;
Keishin Sugawara, Kumamoto, JP;
Tatsufumi Onchi, Kumamoto, JP;
Kazuhiro Komatsu, Kumamoto, JP;
Satoshi Koyanagi, Kumamoto, JP;
Hideo Yoshizaki, Sayama, JP;
Juridical Foundation The Chemo-Sero-Therapeutic Research Institute, Kumamoto, JP;
Kowa Company, Ltd., Nagoya, JP;
Abstract
The present invention relates to a method for purifying a calcium ion-binding protein by cation exchange chromatography. The present invention provide a method for isolating and purifying a calcium ion-binding protein in a simple and efficient manner from a liquid sample containing a calcium ion-binding protein and contaminants without any pretreatment such as addition of a chelating agent. More specifically, the present invention relates to a method for purifying a calcium ion-binding protein which comprises contacting said protein with a cation exchange carrier in the presence of calcium ions to let the said protein be adsorbed to the carrier, and after washing, eluting said protein, and to a calcium ion-binding protein having substantially no contaminants obtained by the method of the present invention.