John Girdner Atwood

Title: John Girdner Atwood - Pioneer in PCR Technology

Introduction

John Girdner Atwood, based in West Redding, CT, is a notable inventor recognized for his significant contributions to the field of biotechnology. With an impressive portfolio of 41 patents, Atwood has established himself as a leader in the development of advanced instruments for polymerase chain reaction (PCR) technology.

Latest Patents

Among Atwood's latest innovations is a Thermal Cycler for PCR, an instrument designed to perform highly accurate PCR processes. This innovative device incorporates an assembly featuring a sample block, several Peltier thermal electric devices, and a heat sink, all meticulously clamped together. The control algorithm within the device adeptly manipulates the current supplied to thermoelectric coolers to regulate the block's dynamic thermal performance, implementing predefined thermal profiles of sample temperature. Notably, the sample temperature is determined through a design-specific model and equations rather than direct measurement, ensuring precision and accuracy. The accompanying control software includes calibration diagnostics that accommodate variations in thermoelectric cooler performance, guaranteeing uniform functionality across different instruments. Additionally, the block/heat sink assembly is interchangeable with others of the same or different design, equipped with an on-board memory device to maintain its precise operating characteristics.

Another breakthrough patent is the Instrument for Monitoring Polymerase Chain Reaction of DNA. This optical instrument adeptly monitors PCR replication within a reaction apparatus, which features a temperature-cycled block housing vials containing reaction ingredients and a dye that fluoresces upon interaction with double-stranded DNA. Through a beam splitter, an excitation beam is directed to the vials, causing the dye to fluoresce. The resulting emission beam is captured by a CCD detector, from which a processor computes the DNA concentration. A unique reference strip with multiple reference emitters provides varying intensity beams, enabling the processor to select the optimal emitter to counteract drift. The exposure time is automatically adjusted to remain within the ideal dynamic ranges of the CCD and processor, while the replaceable beam splitter module and associated optical filters cater to different dye selections.

Career Highlights

Atwood has made significant strides in his career, working with prestigious companies such as the PerkinElmer Corporation and Applied Biosystems, LLC. These experiences have equipped him with a depth of knowledge and expertise, which he has effectively translated into his patented inventions.

Collaborations

Throughout his career, Atwood has collaborated with other notable professionals, including Michael R. Gambini and Edward J. Lakatos. Such collaborations have not only enhanced his innovative capabilities but have also contributed to advancements in PCR technology and related fields.

Conclusion

John Girdner Atwood's dedication to innovation in the biotechnology sector is evident through his substantial number of patents and groundbreaking inventions. His work, particularly in the realm of PCR technology, continues to influence and improve various scientific applications, making significant strides in the accuracy and efficiency of DNA analysis.