Location History:
- Estes Park, CO (US) (2000)
- Lincoln, NE (US) (1988 - 2001)
Company Filing History:
6 out of 730 
3 out of 155 
1 out of 3,435 
Years Active: 1988-2001
Title: Innovations of John A Brumbaugh
Introduction
John A Brumbaugh is a notable inventor based in Lincoln, NE (US). He has made significant contributions to the field of DNA technology, holding a total of 10 patents. His work has advanced the methods of DNA sequencing and detection, showcasing his expertise and innovative spirit.
Latest Patents
One of his latest patents involves DNA sequencing and DNA terminators. This universal terminator includes a heterocycle other than naturally occurring DNA heterocycles such as adenine, cytosine, guanine, and thymine. In this method, the hybridized primer-template is split into four aliquots. In the “A” vial, DNA polymerase is added along with normal amounts of “C”, “G”, and “T” deoxynucleotides, along with a reduced amount of “A” deoxynucleotide and an amount of the universal terminator. This setup ensures that statistically, the universal terminator has less than a one percent chance of being incorporated at sites where a “C”, “G”, or “T” deoxynucleotide should be incorporated, and about a one percent chance of being incorporated at sites where an “A” deoxynucleotide should be incorporated. Similar strategies are followed for the “C”, “G”, and “T” vials, where the amounts of their respective deoxynucleotides are reduced.
Another significant patent is a method for the fluorescent detection of a DNA sequence in real time. This invention relates to detecting a DNA sequence by means of a DNA:DNA hybrid using fluorescence. It eliminates the need for radioactive probes to detect DNA and removes the delay associated with autoradiographic exposure of the X-ray to the radioactive label.
Career Highlights
John A Brumbaugh has worked with esteemed organizations such as the University of Nebraska and Li-Cor, Inc. His career reflects a commitment to advancing scientific knowledge and technology in the field of genetics.
Collaborations
Throughout his career, he has collaborated with notable individuals, including Lyle R Middendorf and Gi Young Jang. These partnerships have contributed to the success of his innovative projects.
Conclusion
John A Brumbaugh's contributions to DNA technology through his patents and collaborations highlight his role as a leading inventor in the field. His work continues to influence advancements
Inventor’s Patent Attorneys refers to legal professionals with specialized expertise in representing inventors throughout the patent process. These attorneys assist inventors in navigating the complexities of patent law, including filing patent applications, conducting patent searches, and protecting intellectual property rights. They play a crucial role in helping inventors secure patents for their innovative creations.