The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Mar. 27, 2001
Filed:
Jul. 21, 1995
Lyle Richard Middendorf, Lincoln, NE (US);
John A. Brumbaugh, Lincoln, NE (US);
Li-Cor, Inc., Lincoln, NE (US);
Abstract
A universal terminator includes a heterocycle other than naturally occuring DNA heterocycles such as adenine, cytosine, guanine and thymine. In terminating strand synthesis, the hybridized primer-template is split into four aliquots. In the “A” vial is added DNA polymerase and normal amounts of “C”, “G”, and “T” deoxynucleotides, along with a reduced amount of “A” deoxynucleotide and an amount of the universal terminator such that statistically the universal terminator has less than a one percent chance of being incorporated at sites where a “C”, “G”, or “T” deoxynucleotide should be incorporated and about a one percent chance of being incorporated at sites where an “A” deoxynucleotide should be incorporated. Similar strategies are followed for the “C”, “G”, and “T” vials, except that the amount of “C”, “G” and “T” deoxynucleotides are reduced for their respective vial.