Company Filing History:
Years Active: 1991-1992
Title: The Innovations of James W. Bloom
Introduction
James W. Bloom is a notable inventor based in Richmond, CA (US). He has made significant contributions to the field of antibody preparation, holding a total of 2 patents. His work focuses on improving the purification processes of antibodies, which are crucial in various medical applications.
Latest Patents
James W. Bloom's latest patents include innovative methods for the removal of protein A from antibody preparations. The first patent describes an antibody preparation that is purified using immobilized protein A, resulting in a product that is substantially free of protein A that may have solubilized during the purification process. This method ensures that the antibodies contain less than 15 ng of protein A per mg of antibody, with a preferred target of less than 1 ng/mg. The low protein A content is achieved by first contacting the antibodies and solubilized protein A with an ion exchange resin, which adsorbs both components. The antibodies and protein A are then sequentially eluted under conditions of increasing ionic strength.
The second patent also focuses on the selective isolation of protein A from an antibody-protein A mixture. This is accomplished by exposing the mixture to an anion exchange material under conditions that allow for the adsorption of both components. The antibodies and protein A are then eluted sequentially under conditions of increasing ionic strength, resulting in antibody preparations that contain less than about 15 ng of protein A per mg of antibody.
Career Highlights
James W. Bloom is associated with Miles Inc., where he has been able to apply his expertise in antibody purification. His work has contributed to advancements in the field, enhancing the quality and efficacy of antibody preparations used in various applications.
Collaborations
Some of his notable coworkers include Melvin F. Wong and Gautam Mitra. Their collaboration has likely fostered an environment of innovation and shared knowledge, furthering the development of effective antibody purification techniques.
Conclusion
James W. Bloom's contributions to the field of antibody preparation through his patents demonstrate his commitment to innovation and improvement in medical applications. His work continues to impact the industry positively, paving the way for advancements in antibody purification processes.