The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
May. 19, 1992
Filed:
Jun. 26, 1991
Applicant:
Inventors:
James W Bloom, Richmond, CA (US);
Melvin F Wong, San Francisco, CA (US);
Gautam Mitra, Kensington, CA (US);
Assignee:
Miles Inc., Elkhart, IN (US);
Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C07K / ; C07K / ; C07K / ;
U.S. Cl.
CPC ...
53038825 ; 530412 ; 530413 ; 530416 ; 530417 ; 5303881 ; 5303871 ; 5303895 ; 435-733 ;
Abstract
Antibody preparation purified using immobilized protein A and yet substantially free of protein A that may have solubilized during the purificaiton process. The antibodies include less than 15 ng protein A per mg of antibody, preferably less than 1 ng/mg. Low protein A content is obtained by first contacting the antibodies and solubilized protein A with an ion exchange resin under conditions to adsorb both. The antibodies and protein A are then sequentially eluted under conditions of increasing ionic strength.