Company Filing History:
Years Active: 1999-2004
Title: Istefo Moisyadi: Innovator in Coffee Plant Biotechnology
Introduction
Istefo Moisyadi is a prominent inventor based in Honolulu, HI (US), known for his significant contributions to the field of biotechnology, particularly in the area of coffee plant research. With a total of five patents to his name, Moisyadi has made strides in understanding and controlling the ripening process of coffee fruit.
Latest Patents
His latest patents focus on purified proteins, recombinant DNA sequences, and processes for controlling the ripening of coffee plants. The invention establishes that coffee fruit ripening is climacteric. It provides techniques to isolate substantially pure RNA from coffee fruit, despite the presence of high levels of phenolic compounds and carbohydrates that typically interfere with clean RNA preparations. The invention includes purified proteins, nucleic acid sequences that code for expression, and recombinant DNA molecules, along with methods for transforming coffee plants to suppress the expression of coffee fruit-expressed ACC synthase and/or ACC oxidase, which are essential for ethylene biosynthesis and the ripening of coffee fruit. By transforming coffee plants with vectors containing these DNA sequences, the expression of the respective enzymes is eliminated, rendering the plants incapable of synthesizing ethylene during the ripening process, while other metabolic aspects remain unaffected. Furthermore, the invention outlines methods for controlling the ripening of coffee fruit from the transformed plants by applying exogenous ethylene.
Career Highlights
Istefo Moisyadi is affiliated with the University of Hawai'i, where he conducts his research and development in biotechnology. His work has garnered attention for its innovative approach to enhancing coffee production and quality.
Collaborations
Some of his notable coworkers include John I Stiles and Kabi Raj Neupane, who have collaborated with him on various research projects.
Conclusion
Istefo Moisyadi's contributions to coffee plant biotechnology exemplify the intersection of innovation and agricultural science. His patents not only advance our understanding of plant biology but also offer practical solutions for improving coffee production.
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