The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Feb. 23, 1999
Filed:
Aug. 12, 1996
John I Stiles, Kaneohe, HI (US);
Istefo Moisyadi, Honolulu, HI (US);
Kabi Raj Neupane, Honolulu, HI (US);
University of Hawaii, Honolulu, HI (US);
Abstract
The invention provides purified proteins, DNA sequences that code on expression therefore and recombinant DNA molecules, including hosts transformed therewith for transforming coffee plants to suppress the expression of enzymes necessary for ethylene synthesis. The DNA sequences and recombinant DNA molecules are characerized in that they code on expression for the enzymes ACC synthase or ACC oxidase that are elements of the pathway for ethylene biosynthesis in coffee plants. Coffee plants are transformed with vectors containing ACC synthase and/or with ACC oxidase DNA sequences that code on expression for the respective mRNA that is antisense to the mRNA for ACC synthase and/or ACC oxidase. The resulting antisense mRNA binds to the respective ACC synthase and/or ACC oxidase mRNA, thereby inactivating the mRNA encoding one or more enzymes in the pathway for ethylene synthesis. The described DNA sequences can also be used to block synthesis of ACC synthase or ACC oxidase using co-suppression. The result in either event is that the transformed plants are incapable of synthesizing ethylne, though other aspects of their metabolism is not affected.