Fufeng Liu

Title: Fufeng Liu: Innovator in Alkaline Protease and Affinity Peptide Library Development

Introduction

Fufeng Liu is a prominent inventor based in Tianjin, China. He has made significant contributions to the field of biochemistry, particularly in the development of alkaline protease mutants and affinity peptide libraries. His innovative work has led to the filing of multiple patents, showcasing his expertise and dedication to advancing scientific knowledge.

Latest Patents

Fufeng Liu holds 2 patents that highlight his innovative approaches. His first patent involves an alkaline protease mutant, along with a gene, engineered strain, preparation method, and application thereof. This method includes extracting genome DNA, performing PCR amplification to obtain a wild-type alkaline protease gene sequence, and mutating the gene through error-prone PCR. The process also involves high-throughput screening to identify highly active alkaline protease genes, followed by DNA shuffling to obtain eight mutant genes with enhanced activity.

His second patent focuses on constructing a novel affinity peptide library for binding immunoglobulin G (IgG) based on a protein affinity model of Protein A. This involves using the Molecular Mechanics—Poisson-Boltzmann surface area (MM/PBSA) method to analyze the human IgG-Protein A complex structure. The resulting affinity peptide library includes heptapeptide and octapeptide structural modes, which are screened through molecular docking and dynamics simulation methods to identify effective IgG purification ligands.

Career Highlights

Fufeng Liu has worked at notable institutions, including Tianjin University of Science and Technology and Shandong Lonct Enzymes Co. Ltd. His experience in these organizations has allowed him to collaborate with other experts in the field and contribute to significant advancements in enzyme technology.

Collaborations

Fufeng Liu has collaborated with colleagues such as Fuping Lu and Yihan Liu. These partnerships have fostered a collaborative environment that encourages innovation and the sharing of ideas.

Conclusion

Fufeng Liu's contributions to the fields of alkaline protease and affinity peptide libraries demonstrate his commitment to scientific advancement. His patents reflect a deep understanding of biochemistry and a drive to innovate. Through his work, he continues to influence the landscape of enzyme research and development.