The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
May. 23, 2023
Filed:
Jul. 31, 2022
Tianjin University of Science and Technology, Tianjin, CN;
Shandong Lonct Enzymes Co. Ltd, Linyi, CN;
Fuping Lu, Tianjin, CN;
Yihan Liu, Tianjin, CN;
Yu Li, Tianjin, CN;
Xingji Wang, Linyi, CN;
Kefen Wang, Linyi, CN;
Wenlong Liu, Linyi, CN;
Fufeng Liu, Tianjin, CN;
Huitu Zhang, Tianjin, CN;
Tianjin University of Science and Technology, Tianjin, CN;
Shandong Lonct Enzymes Co. LTD, Linyi, CN;
Abstract
An alkaline protease mutant, and a gene, engineered strain, a preparation method and application thereof are provided. The method comprises the following steps of extracting genome DNA of, performing PCR amplification to obtain a wild-type alkaline protease gene sequence, mutating the wild-type alkaline protease gene obtained by the amplification through an error-prone PCR, performing high-throughput screening to obtain a plurality of highly active alkaline protease genes, performing DNA shuffling on the highly active alkaline protease genes, and performing screening to obtain eight alkaline protease mutant genes with higher activity.