Frank J Lucas

Title: Frank J Lucas: A Pioneer in Cellular Sample Preparation Innovations

Introduction

Frank J Lucas, an esteemed inventor based in Boca Raton, FL, has made significant strides in the field of biological sample preparation. With a remarkable portfolio of 10 patents, his contributions have considerably advanced methods for intracellular antigen detection, particularly utilizing flow cytometry. His innovative approaches not only streamline processes but also enhance the accuracy and efficiency of cellular analysis.

Latest Patents

Among Frank J Lucas's latest patents is a groundbreaking method and apparatus for preparing cell samples for intracellular antigen detection using flow cytometry. This innovation addresses the traditional challenges associated with sample preparation by eliminating several conventional steps, thereby facilitating automation. The method encompasses the following essential steps: (a) cell-fixation, (b) permeabilization, and (c) staining or labeling of intracellular molecules with detectable probes. Notably, this process is executed without intervening cell-washing steps, with a single washing step occurring post-analysis. The washing utilizes a semi-permeable membrane to effectively filter out smaller interferants while retaining the targeted cells.

Additionally, he has patented an apparatus for biological sample preparation and analysis, which employs a filtration device designed for automated interference removal. This device features a microporous hollow fiber membrane, which retains cells while allowing smaller interferants to pass through. The method incorporates a vacuum source that transports the sample through the membrane, ensuring that the sample is efficiently analyzed.

Career Highlights

Throughout his professional journey, Frank J Lucas has made a mark by collaborating with leading companies such as the Coulter Corporation and Coulter International Corporation. These experiences have allowed him to apply his inventive ideas in practical settings, contributing to advancements in medical technology.

Collaborations

In his pursuit of innovation, Frank has had the privilege of working alongside accomplished professionals like Gerald E Jaffe and James H Carter. Their collaborative efforts have further enriched his inventions, fostering a collaborative spirit that is crucial in driving scientific advancements.

Conclusion

Frank J Lucas stands as a prominent figure in the innovation of biological sample preparation techniques. His inventions, characterized by automation and efficiency, reflect a deep understanding of the intricacies of cellular analysis. As he continues to push the boundaries of scientific inquiry, his work not only benefits research institutions but also enhances the broader field of biomedical science. Frank J Lucas's journey exemplifies the spirit of innovation that is essential for progress in the realm of cellular research.