This inventor holds 13 USPTO granted patents and 3 published patent applications. Top assignees: Florida International University, Clemson University, University of Central Florida Research Foundation Inc.. Active years: 2018-2026.
Company Filing History:
13 out of 602 
1 out of 458 
1 out of 1,364 
Years Active: 2018-2026
Title: Innovations of Fenfei Leng: A Pioneer in DNA Synthesis and Assay Development
Introduction
Fenfei Leng is an accomplished inventor based in Palmetto Bay, Florida, with a remarkable portfolio of 11 patents. His work primarily focuses on the synthesis of DNA molecules and the development of high-throughput screening assays for drug discovery. His innovative approaches have significantly contributed to the fields of molecular biology and biochemistry.
Latest Patents
Among his latest patents, Fenfei Leng has developed a method for the synthesis of DNA molecules in in vitro enzymatic systems. This method allows for the creation of closed circular single-stranded and double-stranded DNA molecules of various sizes. The process includes the removal of unwanted DNA molecules using exonucleases, such as T5 exonuclease and T7 exonuclease. Additionally, he has described a method for converting single-stranded circular DNA molecules into double-stranded circular DNA molecules, which can be utilized in various applications.
Another significant patent involves a high-throughput screening assay designed to identify DNA topoisomerase inhibitors. This novel fluorescence-based assay utilizes T5 exonuclease-amplified DNA cleavage to facilitate the discovery of gyrase poisoning inhibitors. The assay allows for the simultaneous binding of multiple gyrase molecules to a plasmid DNA molecule, forming stable gyrase-DNA cleavage complexes. This innovative approach is suitable for screening large compound libraries to identify inhibitors against human DNA topoisomerase IIα.
Career Highlights
Fenfei Leng has had a distinguished career, contributing to significant advancements in DNA synthesis and assay development. His work has been recognized in various scientific communities, and he has established himself as a leading figure in his field.
Collaborations
Throughout his career, Fenfei Leng has collaborated with notable colleagues, including Zifang Deng and Xiaoduo Zhi. These collaborations have further enhanced the impact of his research and innovations.
Conclusion
Fenfei Leng's contributions to the field of DNA synthesis and assay development exemplify his innovative spirit and dedication to scientific advancement. His patents and collaborative efforts continue to influence research in molecular biology and drug discovery.