The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Mar. 08, 2022
Filed:
Jun. 10, 2021
Applicants:
Fenfei Leng, Palmetto Bay, FL (US);
Zifang Deng, Miami, FL (US);
Inventors:
Fenfei Leng, Palmetto Bay, FL (US);
Zifang Deng, Miami, FL (US);
Assignee:
THE FLORIDA INTERNATIONAL UNIVERSITY BOARD OF TRUSTEES, Miami, FL (US);
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12Q 1/533 (2006.01); G01N 21/64 (2006.01); C12Q 1/44 (2006.01);
U.S. Cl.
CPC ...
C12Q 1/533 (2013.01); C12Q 1/44 (2013.01); G01N 21/6428 (2013.01); G01N 2021/6439 (2013.01); G01N 2500/04 (2013.01); G01N 2500/20 (2013.01);
Abstract
The present invention provides assays and methods for studying DNA topology and topoisomerases. The assays and methods utilize a circular plasmid DNA comprising one or more hairpin structures and the ability of T5 exonuclease (T5E) to digest the circular plasmid DNA in a specific configuration. The assays and methods can be used as a high throughput screening for inhibitors of, for example, DNA gyrases and DNA topoisomerases I for anticancer drug and antibiotics discovery.