Eva-Maria Tombak

Title: **Eva-Maria Tombak: Innovator in Mammalian Cell Line Development**

Introduction

Eva-Maria Tombak, an accomplished inventor based in Õssu, Estonia, has made significant contributions to the field of cellular biology through her innovative approaches in developing stable mammalian cell lines. With a total of two patents to her name, she exemplifies the spirit of ingenuity and scientific advancement in modern research.

Latest Patents

Eva-Maria's latest patent is titled "Platform for Developing Stable Mammalian Cell Lines." This patent provides innovative methods and landing pad constructs designed for the generation of parental cell lines suitable for targeted integration. The method involves introducing binding sites of the BPV1 E2 protein to landing pad vectors, enabling the expressed BPV1 E2 protein to locate the vector in transcriptionally active regions within the genome. This meticulous process allows for the selection of cells with high expression levels of reporter genes, which are crucial for the next stages of developing cell lines that express other recombinant proteins via recombination-mediated cassette exchange (RMCE). Additionally, the landing pad constructs incorporate recombination target sites for site-specific recombinases, allowing for a more effective replacement with gene-of-interest expression constructs. Ultimately, this innovative platform enables the generation of producer cell lines with significantly less effort compared to traditional methods reliant on random integration.

Career Highlights

Eva-Maria Tombak is currently affiliated with Icosagen Cell Factory OÜ, a company dedicated to advancing cell line technology. Her role involves leading research initiatives that focus on the intersection of biotechnology and cellular engineering, making impactful strides in both academic and commercial arenas.

Collaborations

During her career, Eva-Maria has collaborated with esteemed colleagues, including Mart Ustav, Jr. and Kadri Õunap. These partnerships have fostered an environment of shared knowledge and innovative problem-solving, further enhancing the scope and impact of their research endeavors.

Conclusion

Eva-Maria Tombak stands out as a notable inventor in the scientific community, particularly in the realm of mammalian cell line development. Her groundbreaking patents and collaborative efforts highlight her commitment to advancing biotechnology and improving methodologies that significantly benefit industry practices and research outcomes. With her continued contributions, the future of cellular biotechnology looks promising, driven by the innovations of dedicated inventors like Eva-Maria.