The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jun. 27, 2023
Filed:
Oct. 17, 2019
Icosagen Cell Factory Oü, Tartumaa, EE;
Kadri Õunap, Õssu, EE;
Eva-Maria Tombak, Õssu, EE;
Mart Toots, Õssu, EE;
Madis Jakobson, Õssu, EE;
Mart Ustav, Jr., Õssu, EE;
Kerttu Murumets, Õssu, EE;
Urve Toots, Õssu, EE;
Andres Männik, Õssu, EE;
Mart Ustav, Sr., Õssu, EE;
Icosagen Cell Factory OÜ, Õssu, EE;
Abstract
This disclosure provides methods and landing pad constructs for generation of parental cell lines suitable for targeted integration. A method is provided by the parental cell line development; this is, the introduction of binding sites of BPV1 E2 protein to landing pad vectors so that expressed BPV1 E2 protein could locate the vector to transcriptionally active region in the genome. Cells with high expression level of reporter genes are selected for the next stage and will be used in the development of cell lines expressing another recombinant protein by recombination mediated cassette exchange (RMCE). Landing pad constructs include recombination target sites for site-specific recombinases, and therefore, it could be replaced with gene-of-interest expression construct containing the same set of recombination target sites. This yields the generation of producer cell lines with less effort compared to traditional cell line development by random integration.