David P Hornby

Title: Innovations in RNA and Polynucleotide Separation by David P Hornby

Introduction

David P Hornby, an accomplished inventor based in Windes, Cheshire, GB, holds four patents that significantly contribute to the field of molecular biology. His innovative work particularly focuses on enhancing the methods for separating polynucleotides, such as DNA and RNA, which are vital for various genetic analyses.

Latest Patents

Hornby's latest patents include revolutionary methods that enhance the separation of polynucleotides. One notable patent outlines a technique for liquid chromatographic separation of polynucleotides. This invention involves a method that applies the mixture of polynucleotides to a polymeric separation medium characterized by non-polar surfaces which are largely devoid of interfering multivalent cations. The elution process incorporates an organic solvent and counter ion agent, and ideally, the separation is enhanced at high temperatures to effectively denature secondary structures within the polynucleotides.

Another significant patent introduced by Hornby addresses a method and system for RNA analysis through Matched Ion Polynucleotide Chromatography. This method facilitates size-based segregation of RNA molecules by applying the RNA mixture to a specific polymeric medium and using a mobile phase that contains a counterion reagent along with an organic component. The preferred surfaces are designed to limit interference from multivalent cations, and the separation process can handle RNA molecules ranging from 100 to 20,000 nucleotides. Improved segregation results are achieved through the use of chromatography columns with diameters greater than about 5 mm, utilizing separation media such as beads and monolithic columns.

Career Highlights

Throughout his career, David P Hornby has made notable strides in the field of biotechnology, particularly while working with Transgenomic, Inc. His contributions are highly regarded within academic and commercial circles seeking precise methods for molecular separation and analysis.

Collaborations

Hornby has collaborated with fellow innovators, including Christopher P Hanna and Douglas T Gjerde. Together, they have contributed to advancements in the field of molecular biology by developing effective methods and systems for the analysis and separation of polynucleotides.

Conclusion

David P Hornby's innovative approaches to the separation of polynucleotides and RNA analysis represent substantial advancements in biotechnology. His patents not only enhance the efficiency and accuracy of genetic analysis but also pave the way for further research and development in this critical area of science. As the biotechnology landscape continues to evolve, Hornby's work remains integral to enabling researchers and companies to push boundaries in molecular biology.