The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Nov. 21, 2006
Filed:
Nov. 16, 2000
Douglas T. Gjerde, Saratoga, CA (US);
Robert M. Haefele, Campbell, CA (US);
Paul D. Taylor, Gilroy, CA (US);
Christopher P. Hanna, Greenfield, MA (US);
Alezander I. Kuklin, Collegeville, PA (US);
David P. Hornby, Windes Cheshire, GB;
Douglas T. Gjerde, Saratoga, CA (US);
Robert M. Haefele, Campbell, CA (US);
Paul D. Taylor, Gilroy, CA (US);
Christopher P. Hanna, Greenfield, MA (US);
Alezander I. Kuklin, Collegeville, PA (US);
David P. Hornby, Windes Cheshire, GB;
Transgenomic, Inc., Omaha, NE (US);
Abstract
In one aspect, the invention provides a method for separating a mixture of polynucleotides, such as DNA or RNA, including (a) applying the mixture to a polymeric separation medium having non-polar surfaces, wherein the surfaces are characterized by being substantially free from multivalent cations, such as metal ions, which are free to interfere with polynucleotide separation, and (b) eluting the mixture with a mobile phase containing organic solvent and counter ion agent. In the separation of single-stranded polynucleotides, improved separation is obtained at a temperature effective to fully denature secondary structure within the polynucleotides.