Company Filing History:
2 out of 143 
2 out of 6 
1 out of 1 Years Active: 1995-1998
Title: David A Mead: Innovator in Molecular Biology
Introduction
David A Mead is a prominent inventor based in Madison, WI (US), known for his significant contributions to molecular biology. With a total of 4 patents, he has made remarkable advancements in the field, particularly in the area of DNA manipulation and cloning techniques.
Latest Patents
One of his latest patents focuses on the direct cloning of PCR amplified nucleic acids. This invention describes methods for producing recombinant DNA molecules from suitable host vectors and nucleic acids subjected to 3'-terminal transferase activity. The method utilizes single 3'-deoxy-adenosine monophosphate (dAMP) residues attached to the 3' termini of PCR generated nucleic acids. Vectors are prepared with recognition sequences that afford single 3'-terminal deoxy-thymidine monophosphate (dTMP) residues upon reaction with a suitable restriction enzyme. This innovation allows PCR generated copies of genes to be directly cloned into the vectors without the need for preparing primers with suitable restriction sites. Additionally, the invention includes associated plasmid vectors and kits for implementing these methods.
Another significant patent involves methods and materials for restriction endonuclease applications. This invention is directed towards the quasi-random and complete fragmentation of DNA using restriction endonuclease reagents capable of cutting DNA at a dinucleotide sequence. It also covers methods for labeling DNA using template-specific oligonucleotides, which can be utilized for shotgun cloning, sequencing of DNA, epitope mapping, and anonymous primer cloning, all using fragments generated by this innovative method.
Career Highlights
Throughout his career, David A Mead has worked with notable companies such as Invitrogen Corporation and Molecular Biology Resources, Inc. His work has significantly impacted the field of molecular biology, particularly in the development of techniques that enhance DNA manipulation.
Collaborations
David has collaborated with esteemed colleagues, including Corinna Herrnstadt and Joseph M Fernandez, contributing to various projects that advance the understanding and application of molecular biology techniques.
Conclusion
David A Mead's innovative work in molecular biology, particularly in DNA cloning and manipulation, has led to significant advancements in the field. His contributions continue to influence research and applications in biotechnology and genetics.