The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Feb. 18, 1997

Filed:

Dec. 22, 1994
Applicant:
Inventors:

David Mead, Madison, WI (US);

Neela Swaminathan, Madison, WI (US);

Assignee:

Molecular Biology Resources, Inc., Milwaukee, WI (US);

Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12Q / ; C12P / ; C07H / ; C12N / ;
U.S. Cl.
CPC ...
435-6 ; 435 911 ; 435 912 ; 4351721 ; 435810 ; 536 231 ; 536 2432 ; 536 2433 ; 536 253 ; 536 2532 ; 935 77 ; 935 78 ;
Abstract

The present invention is directed to materials and methods for the quasi-random and complete fragmentation of DNA using restriction endonuclease reagents capable of cutting DNA at a dinucleotide sequence. The invention is also directed to methods for labeling DNA using template-specific oligonucleotides, for shotgun cloning, for sequencing of DNA, for epitope mapping and for anonymous primer cloning, all using fragments of DNA generated by the method of the present invention.


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