Daniel J Mandell

Title: The Innovative Contributions of Daniel J Mandell

Introduction

Daniel J Mandell is a prominent inventor based in Brookline, MA (US). He has made significant contributions to the field of biosensors and enzymatic activity, holding a total of 6 patents. His work has implications in various applications, including metabolic engineering and transcription control.

Latest Patents

One of Mandell's latest patents focuses on small molecule biosensors. These biosensors can be utilized in applications ranging from metabolic engineering to orthogonal control of transcription. They are produced based on a ligand-binding domain (LBD) that can be applied to any target molecule. The LBD is fused to either a fluorescent protein or a transcriptional activator and is designed to accumulate only in cells containing the target ligand. This method has been successfully illustrated by developing biosensors for digoxin and progesterone. The addition of ligand to cells expressing a biosensor activates transcription in yeast, mammalian cells, and plants, achieving a dynamic range of up to about 100-fold or more. These biosensors are instrumental in improving the biotransformation of pregnenolone to progesterone in yeast and regulating CRISPR activity in mammalian cells.

Another notable patent involves a stabilized DNase I polypeptide containing a non-standard amino acid. This polypeptide maintains enzymatic activity even under harsh conditions, such as reducing environments. The stabilized DNase I polypeptide exhibits higher enzymatic activity in these conditions compared to a corresponding DNase I polypeptide without non-standard amino acids. This patent also includes polynucleotides encoding the stabilized DNase I polypeptide, cells for expressing and producing it, and methods for its use.

Career Highlights

Mandell has worked with prestigious institutions such as Harvard College and the University of Washington. His innovative research has positioned him as a key figure in the development of advanced biosensing technologies.

Collaborations

Throughout his career, Mandell has collaborated with notable individuals, including George M Church and Christopher John Gregg. These collaborations have further enhanced the impact of his work in the scientific community.

Conclusion

Daniel J Mandell's contributions to the fields of biosensors and enzymatic activity demonstrate his innovative spirit and dedication to advancing scientific knowledge. His patents reflect a commitment to developing technologies that have the potential to transform various applications in biology and medicine.