Company Filing History:
Years Active: 1995-1998
Title: Innovations of Christopher L Chio
Introduction
Christopher L Chio is an accomplished inventor based in Kalamazoo, MI (US). He has made significant contributions to the field of biotechnology, particularly in the synthesis of genes related to dopamine receptors. With a total of two patents to his name, Chio's work has the potential to impact various areas of medical research and treatment.
Latest Patents
Chio's latest patents include a synthetic gene for D4 dopamine receptors. This invention involves a modified gene coding for the human D4 dopamine receptor, synthesized through chemo-enzymatic methods. The nucleotide sequence of the D4 dopamine receptor gene was altered to reduce the G+C content and eliminate intronic sequences, while preserving the published amino acid sequence. By employing gene splicing through overlap extension and PCR amplification of long oligonucleotides, three synthetic fragments of approximately 400 base pairs each were amplified, leading to the assembly of the full-length gene. Stable expression of this gene has been successfully achieved in CHO-K1 cells using an inducible expression system, as well as in HEK293 cells. Another patent focuses on a binding assay utilizing the synthetic gene for D4 dopamine receptors, showcasing the versatility and applicability of his research.
Career Highlights
Chio is currently associated with The Upjohn Co., where he continues to innovate and contribute to advancements in biotechnology. His work is characterized by a strong emphasis on genetic engineering and its applications in pharmacology.
Collaborations
Chio collaborates with Rita M Huff, leveraging their combined expertise to further their research initiatives.
Conclusion
Christopher L Chio's innovative work in the synthesis of dopamine receptor genes exemplifies the potential of biotechnology to transform medical research. His contributions are paving the way for future advancements in the field.