The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Nov. 21, 1995
Filed:
Jul. 01, 1993
Christopher L Chio, Kalamazoo, MI (US);
Rita M Huff, Kalamazoo, MI (US);
The Upjohn Company, Kalamazoo, MI (US);
Abstract
A modified gene coding for the human D4 dopamine receptor has been synthesized by chemo-enzymatic methods. The nucleotide sequence of the D4 dopamine receptor gene was oh:raged to reduce the G+C content and to eliminate intronic sequences, while maintaining the published amino acid sequence. Using gene splicing by overlap extension and PCR amplification of long oligonucleotides (>200 bases), 3 synthetic fragments of about 400 base pairs each were amplified, from which the full length gene was assembled. Stable expression of this gene has been achieved in CHO-K1 cells, using an inducible expression system, and in HEK293 cells.