Company Filing History:
Years Active: 1995-1998
Title: Innovations of Rita M Huff
Introduction
Rita M Huff is a notable inventor based in Kalamazoo, MI (US). She has made significant contributions to the field of genetic engineering, particularly in the synthesis of modified genes. With a total of 2 patents to her name, her work has implications for understanding dopamine receptors and their functions.
Latest Patents
Rita's latest patents include a synthetic gene for D4 dopamine receptors. This invention involves a modified gene coding for the human D4 dopamine receptor, synthesized through chemo-enzymatic methods. The nucleotide sequence of the D4 dopamine receptor gene was altered to reduce the G+C content and eliminate intronic sequences, while maintaining the published amino acid sequence. Using gene splicing by overlap extension and PCR amplification of long oligonucleotides greater than 200 bases, three synthetic fragments of about 400 base pairs each were amplified, from which the full-length gene was assembled. Stable expression of this gene has been achieved in CHO-K1 cells, utilizing an inducible expression system, and in HEK293 cells. Another patent focuses on a binding assay employing the synthetic gene for D4 dopamine receptors, showcasing her innovative approach to genetic research.
Career Highlights
Rita M Huff is currently associated with The Upjohn Company, where she continues to advance her research in genetic engineering. Her work has garnered attention for its potential applications in pharmacology and neuroscience.
Collaborations
Rita collaborates with Christopher L Chio, contributing to the advancement of their shared research interests.
Conclusion
Rita M Huff's innovative work in the synthesis of modified genes has positioned her as a significant figure in genetic research. Her contributions continue to influence the understanding of dopamine receptors and their applications in medicine.