Company Filing History:
Years Active: 2002-2010
Title: **Ana Mildner: Innovator in Enzyme Refolding Techniques**
Introduction
Ana Mildner is a prominent inventor based in Kalamazoo, MI, recognized for her innovative contributions to the field of biochemistry. With a total of three patents to her name, Ana has made significant strides in the area of enzyme refolding, which is crucial for various biochemical applications.
Latest Patents
One of Ana Mildner's latest patents, titled "Method for Refolding Enzymes," provides a method for efficient recombinant expression, refolding, and purification of Beta-site APP cleaving enzyme (BACE) polypeptides. This groundbreaking invention outlines a step-by-step procedure that includes expressing a recombinant construct in bacteria, dissolving inclusion bodies with a denaturant at high pH in the presence of a reducing agent, and finally, allowing the diluted sample to incubate at low temperatures until the recombinant BACE polypeptide correctly folds into an active enzyme. This patent enhances the efficiency and effectiveness of enzyme production, crucial for further research and therapies.
Career Highlights
Ana has built her career at esteemed companies such as Pharmacia & Upjohn Company and Elan Pharmaceuticals, Inc. Her work in these pharmaceutical giants has not only honed her skills in biochemistry but has also provided her with a platform to bring her innovative ideas to fruition. Ana's contributions have improved enzyme production techniques, making her a respected figure in the scientific community.
Collaborations
Throughout her career, Ana has had the privilege of working alongside notable coworkers like Robert L. Heinrikson and Alfredo Giuseppe Tomasselli. These collaborations have enabled her to exchange ideas and push the boundaries of enzyme technology, further enhancing her inventions and the impact of her work in the field.
Conclusion
Ana Mildner exemplifies the spirit of innovation in the life sciences sector. Her patents in enzyme refolding not only showcase her expertise but also highlight her commitment to advancing biochemistry. As she continues her work, her contributions are sure to influence the future of enzyme technologies and their applications in various industries.