The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jun. 08, 2010
Filed:
Feb. 07, 2007
Alfredo Tomasselli, Kalamazoo, MI (US);
Robert Heinrikson, Plainwell, MI (US);
Donna Paddock, Kalamazoo, MI (US);
Ana Mildner, Kalamazoo, MI (US);
Thomas Emmons, Portage, MI (US);
Alfredo Tomasselli, Kalamazoo, MI (US);
Robert Heinrikson, Plainwell, MI (US);
Donna Paddock, Kalamazoo, MI (US);
Ana Mildner, Kalamazoo, MI (US);
Thomas Emmons, Portage, MI (US);
Elan Pharmaceuticals, Inc., South San Francisco, CA (US);
Abstract
The invention provides methods for efficient recombinant expression, refolding, and purification of Beta-site APP cleaving enzyme (BACE) polypeptides. In various aspects, the method includes the steps of expressing a recombinant construct in bacteria, dissolving inclusion bodies with a denaturant at high pH in the presence of a reducing agent, diluting the solubilized BACE polypeptide in an aqueous solution at a temperature of about 1° C. to 15° C., and incubating the diluted sample at a temperature of about 4° C. to 15° C. until the recombinant BACE polypeptide folds into an active enzyme.