The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jun. 23, 2015
Filed:
Aug. 25, 2009
Ping Tsui, Berwyn, PA (US);
Lei Shi, Radnor, PA (US);
Jin LU, Radnor, PA (US);
John Wheeler, Radnor, PA (US);
Brian Whitaker, Radnor, PA (US);
Lionella Borozdina-birch, San Diego, CA (US);
Juan C. Almagro, Radnor, PA (US);
Bernard Amegadzie, Radnor, PA (US);
Mark Tornetta, Radnor, PA (US);
Ramachandra Reddy, Radnor, PA (US);
David M. Knight, Radnor, PA (US);
Jinquan Luo, Radnor, PA (US);
Raymond W. Sweet, Radnor, PA (US);
Qiang Chen, San Diego, CA (US);
Ping Tsui, Berwyn, PA (US);
Lei Shi, Radnor, PA (US);
Jin Lu, Radnor, PA (US);
John Wheeler, Radnor, PA (US);
Brian Whitaker, Radnor, PA (US);
Lionella Borozdina-Birch, San Diego, CA (US);
Juan C. Almagro, Radnor, PA (US);
Bernard Amegadzie, Radnor, PA (US);
Mark Tornetta, Radnor, PA (US);
Ramachandra Reddy, Radnor, PA (US);
David M. Knight, Radnor, PA (US);
Jinquan Luo, Radnor, PA (US);
Raymond W. Sweet, Radnor, PA (US);
Qiang Chen, San Diego, CA (US);
Janssen Biotech, Inc., Horsham, PA (US);
Abstract
Described and claimed herein are combinatorial synthetic Fab libraries displayed on a phage pIX protein. The libraries were built on scaffolds representing the most frequently used genes in human antibodies, which were diversified to mirror the variability of natural antibodies. After selection using a diverse panel of proteins, numerous specific and high-affinity Fabs were isolated. By a process called in-line maturation the affinity of some antibodies was improved up to one hundred-fold yielding low pM binders suitable for in vivo use. This work thus demonstrates the feasibility of displaying complex Fab libraries as pIX-fusion proteins for antibody discovery and lays the foundations for studies on the structure-function relationship of antibodies.