The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Apr. 07, 2015
Filed:
Jan. 24, 2014
Wyeth Llc, Madison, NJ (US);
Yonghui Yuan, Tappan, NY (US);
Mark Ruppen, Garnerville, NY (US);
Wei-Qiang Sun, Morristown, NJ (US);
Ling Chu, Suffern, NY (US);
John Simpson, Upper Nyack, NY (US);
James Patch, Cornwall on Hudson, NY (US);
Justin Keith Moran, Valley Cottage, NY (US);
Pamela Fink Charbonneau, New City, NY (US);
Wyeth LLC, New York, NY (US);
Abstract
A shortened process for producing a solution containing substantially purified capsular polysaccharides from a cellularlysate broth is described. Ultrafiltering and diafiltering a clarifiedlysate followed by pH adjustment to less than 4.5, preferably about 3.5, precipitated at least 98% of the protein in the solution without seriously affecting polysaccharide yield. Furthermore, following ultrafiltration and diafiltration and acidification to a pH of less than 4.5, filtration using activated carbon precipitated at least 90% of remaining protein without seriously affecting polysaccharide yield. Exemplary, non-limitingserotypes that can be purified using the shortened process of the invention are 1, 4, 5, 6A, 6B, 7F, 9V, 14, 18C, 19A, 19F, and 23F. In one embodiment, thecells are lysed using deoxycholate sodium (DOC), while in another embodiment the lytic agent is a non-animal derived lytic agent such as N-lauryl sarcosine sodium (NLS).