The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Oct. 21, 2014
Filed:
Dec. 17, 2010
Kazumi Kitta, Tsukuba, JP;
Satoshi Furui, Tsukuba, JP;
Junichi Mano, Tsukuba, JP;
Yasuyuki Matsuoka, Atsugi, JP;
Shinichiro Arami, Atsugi, JP;
Megumi Sato, Atsugi, JP;
Hiroyuki Haraguchi, Atsugi, JP;
Youichi Kurimoto, Atsugi, JP;
Shinjiro Imai, Fujimino, JP;
Keiko Tanaka, Fujimino, JP;
Kazumi Kitta, Tsukuba, JP;
Satoshi Furui, Tsukuba, JP;
Junichi Mano, Tsukuba, JP;
Yasuyuki Matsuoka, Atsugi, JP;
Shinichiro Arami, Atsugi, JP;
Megumi Sato, Atsugi, JP;
Hiroyuki Haraguchi, Atsugi, JP;
Youichi Kurimoto, Atsugi, JP;
Shinjiro Imai, Fujimino, JP;
Keiko Tanaka, Fujimino, JP;
Nippon Flour Mills Co., Ltd, Tokyo, JP;
Nisshin Seifun Group Inc, Tokyo, JP;
Abstract
Disclosed are: a method for detecting common wheat among from wheat varieties contained in a sample of interest such as a food raw material or a processed food specifically, with high sensitivity, and in a qualitative and/or quantitative manner; a method for discriminating between common wheat and a wheat variety other than common wheat (e.g., durum wheat) contained in a food raw material or a processed food and detecting the common wheat in a qualitative and/or quantitative manner; and a primer set, a nucleic acid probe, and a detection kit, each of which can be used in the methods employing a PCR method. Specifically disclosed are: a method for detecting the occurrence of common wheat in a sample of interest, which comprises carrying out a PCR method using a nucleic acid extracted from the sample as a template and using a primer comprising the nucleotide sequence represented by SEQ ID NO:5 and a primer comprising the nucleotide sequence represented by SEQ ID NO:6 and detecting the occurrence of a PCR amplification product; and a method for detecting the occurrence of common wheat in a sample of interest, which comprises carrying out a quantitative PCR method using a nucleic acid extracted from the sample as a template and using a primer comprising the nucleotide sequence represented by SEQ ID NO:5, a primer comprising the nucleotide sequence represented by SEQ ID NO:6 and a nucleic acid probe comprising the nucleotide sequence represented by SEQ ID NO:11 and detecting the occurrence of common wheat qualitatively and/or quantitatively.