The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Oct. 21, 2014
Filed:
Jun. 23, 2009
Thomas S. Wehrman, Mountain View, CA (US);
Chin Yee Loh, Kensington, CA (US);
Mahesh Mathrubutham, Cupertino, CA (US);
Keith R. Olson, Pleasanton, CA (US);
Thomas S. Wehrman, Mountain View, CA (US);
Chin Yee Loh, Kensington, CA (US);
Mahesh Mathrubutham, Cupertino, CA (US);
Keith R. Olson, Pleasanton, CA (US);
DiscoveRx Corporation, Fremont, CA (US);
Abstract
Methods and genetic constructs are provided for detecting the binding of nuclear hormone receptors to a coactivator/corepressor. The methods employ enzyme fragment complementation using fragments of β-galactosidase as the detection system. Cells are transformed to express the large fragment of β-galactosidase fused to a member of the complex with NHR for initiation of transcription and have it localized in the nucleus and to express the small fragment of β-galactosidase fused to the nuclear hormone receptor for binding to the member upon stimulation with a ligand.