The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Sep. 02, 2014

Filed:

Feb. 04, 2005
Applicants:

Sami Saribas, Philadelphia, PA (US);

David Hakes, Willow Grove, PA (US);

Scott Willett, Doylestown, PA (US);

Karl F. Johnson, Hatboro, PA (US);

Daniel James Bezila, Quakertown, PA (US);

Shawn Defrees, North Wales, PA (US);

Inventors:

Sami Saribas, Philadelphia, PA (US);

David Hakes, Willow Grove, PA (US);

Scott Willett, Doylestown, PA (US);

Karl F. Johnson, Hatboro, PA (US);

Daniel James Bezila, Quakertown, PA (US);

Shawn DeFrees, North Wales, PA (US);

Assignee:

Novo Nordisk A/S, Bagsvaerd, DK;

Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12P 21/06 (2006.01); C12N 9/10 (2006.01);
U.S. Cl.
CPC ...
C12N 9/1048 (2013.01);
Abstract

The present invention provides methods of refolding mammalian glycosyltransferases that have been produced in bacterial cells, and methods to use such refolded glycosyltransferases, including glycosyltransferase mutants that have enhanced ability to be refolded. The invention also provides methods of refolding more than one glycosyltransferase in a single vessel, methods to use such refolded glycosyltransferases, and reaction mixtures comprising the refolded glycosyltransferases.


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