The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Nov. 26, 2013
Filed:
Apr. 14, 2010
Brian L. Frey, Madison, WI (US);
April L. Jue, Madison, WI (US);
Casey J. Krusemark, Woodside, CA (US);
Lloyd M. Smith, Madison, WI (US);
Joshua J. Coon, Middleton, WI (US);
Brian L. Frey, Madison, WI (US);
April L. Jue, Madison, WI (US);
Casey J. Krusemark, Woodside, CA (US);
Lloyd M. Smith, Madison, WI (US);
Joshua J. Coon, Middleton, WI (US);
Wisconsin Alumni Research Foundation, Madison, WI (US);
Abstract
The present invention provides methods for enhancing the fragmentation of peptides for mass spectrometry by modifying the peptides with a tagging reagent containing a functional group, such as a tertiary amine, having a greater gas-phase basicity than the amide backbone of the peptide. These high gas-phase basicity functional groups are attached to a peptide by reacting the tagging reagent to one or more available carboxylic acid groups of the peptide. Linking these high gas-phase functional groups to the peptides leads to higher charge state ions from electrospray ionization mass spectrometry (ESI-MS), which fragment more extensively during fragmentation techniques, particularly non-ergodic fragmentation techniques such as electron capture dissociation (ECD) and electron transfer dissociation (ETD).