The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Dec. 25, 2012
Filed:
Apr. 25, 2003
Didier Trono, Collonge, CH;
Francois-loic Cosset, Lyons, FR;
Virginie Sandrin, Lyons, FR;
Bertrand Boson, Lyons, FR;
Didier Negre, Lyons, FR;
Patrick Salmon, Arenthon, FR;
Didier Trono, Collonge, CH;
Francois-Loic Cosset, Lyons, FR;
Virginie Sandrin, Lyons, FR;
Bertrand Boson, Lyons, FR;
Didier Negre, Lyons, FR;
Patrick Salmon, Arenthon, FR;
Institut National de la Sante et de la Recherche Medicale (INSERM), Paris, FR;
Institut Clayton de la Recherche, Geneva, CH;
Abstract
The present invention provides improved chimeric glycoproteins (GPs) and improved lentiviral vectors pseudotyped with those glycoproteins. Also provided are methods and compositions for making such glycoproteins and vectors, and improved methods of in vitro and in vivo transduction of cells with such vectors. Improved chimeric GPs encode the extracellular and transmembrane domains of GALV or RD114 GPs fused to the cytoplasmic tail of MLV-A GP. Vectors pseudotyped with these GAL V/TR and RD 114/TR GP chimeras have significantly higher titers than vectors coated with the parental GPs. Additionally, RD114/TR-pseudotyped vectors are efficiently concentrated and are resistant to inactivation induced by the complement of both human and macaque sera. RD114 GP-pseudotyped lentiviral vectors have particular utility for in vivo gene transfer applications.