The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Feb. 22, 2011
Filed:
Mar. 05, 2008
Takahiro Kikawada, Ohwashi 1-2, Tsukuba, Ibaraki 305-8634, JP;
Takashi Okuda, Ohwashi 1-2, Tsukuba, Ibaraki 305-8634, JP;
Masahiko Watanabe, Ohwashi 1-2, Tsukuba, Ibaraki 305-8634, JP;
Ayako Saito, Ohwashi 1-2, Tsukuba, Ibaraki 305-8634, JP;
Yasushi Kanamori, Ohwashi 1-2, Tsukuba, Ibaraki 305-8634, JP;
Yuichi Nakahara, Ohwashi 1-2, Tsukuba, Ibaraki 305-8634, JP;
Takahiro Kikawada, Ohwashi 1-2, Tsukuba, Ibaraki 305-8634, JP;
Takashi Okuda, Ohwashi 1-2, Tsukuba, Ibaraki 305-8634, JP;
Masahiko Watanabe, Ohwashi 1-2, Tsukuba, Ibaraki 305-8634, JP;
Ayako Saito, Ohwashi 1-2, Tsukuba, Ibaraki 305-8634, JP;
Yasushi Kanamori, Ohwashi 1-2, Tsukuba, Ibaraki 305-8634, JP;
Yuichi Nakahara, Ohwashi 1-2, Tsukuba, Ibaraki 305-8634, JP;
Other;
Abstract
There are provided trehalose transporter gene and a method of introducing trehalose into cells by using the gene. Candidates for the trehalose transporter genes were searched inEST, resulting in being obtained cDNA designated as Tret1. Tret1 encodes a 504 amino acid protein with 12 trans-membrane structures. Tret1 expression was induced by desiccation stress and predominant in the fat body. Functional expression of TRET1 inoocytes showed that transport activity was specific for trehalose and independent of extracellular pH and electrochemical membrane potential. The direction of transport of TRET1 was reversible depending on the concentration gradient of trehalose. Apparent Km and Vmax of TRET1 for trehalose were extraordinarily high values. These results indicate that TRET1 is a facilitated, high-capacity trehalose-specific transporter. Tret1 is widespread in insects. Furthermore, TRET1 conferred trehalose permeability upon cells including those of vertebrates as well as insects.