The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jul. 17, 2007
Filed:
Jan. 28, 2004
Yi-ju Chen, New Haven, CT (US);
John H. Leamon, Guilford, CT (US);
Kenton L. Lohman, Guilford, CT (US);
Michael T. Ronan, New Haven, CT (US);
Jonathan M. Rothberg, Guilford, CT (US);
Maithreyan Srinivasan, Branford, CT (US);
Michael P. Weiner, Guilford, CT (US);
Yi-Ju Chen, New Haven, CT (US);
John H. Leamon, Guilford, CT (US);
Kenton L. Lohman, Guilford, CT (US);
Michael T. Ronan, New Haven, CT (US);
Jonathan M. Rothberg, Guilford, CT (US);
Maithreyan Srinivasan, Branford, CT (US);
Michael P. Weiner, Guilford, CT (US);
454 Life Sciences Corporation, Branford, CT (US);
Abstract
This invention relates to methods of sequencing DNA. More specifically, this invention relates to methods of sequencing both the sense and antisense strands of DNA through the use of blocked and unblocked sequencing primers. In brief, these methods include the steps of annealing an unblocked primer to a first strand of nucleic acid; annealing a second blocked primer to a second strand of nucleic acid; elongating the nucleic acid along the first strand with a polymerase; terminating the first sequencing primer; deblocking the second primer; and elongating the nucleic acid along the second strand.