The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Apr. 20, 2021

Filed:

Jan. 16, 2019
Applicant:

Roche Molecular Systems, Inc., Pleasanton, CA (US);

Inventors:

Jan Berka, New Haven, CT (US);

Yi-Ju Chen, New Haven, CT (US);

John H. Leamon, Guilford, CT (US);

Steven Lefkowitz, Branford, CT (US);

Kenton L. Lohman, Guilford, CT (US);

Vinod B. Makhijani, Guilford, CT (US);

Jonathan M. Rothberg, Guilford, CT (US);

Gary J. Sarkis, Guilford, CT (US);

Maithreyan Srinivasan, Branford, CT (US);

Michael P. Weiner, Guilford, CT (US);

Assignee:

Roche Molecular Systems, Inc., Pleasanton, CA (US);

Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
C12Q 1/68 (2018.01); B01L 3/00 (2006.01); C07H 21/00 (2006.01); C12N 15/10 (2006.01); G01N 21/25 (2006.01); G01N 21/64 (2006.01); G01R 33/12 (2006.01); C12Q 1/686 (2018.01); C12Q 1/6834 (2018.01); C12Q 1/6844 (2018.01); C12Q 1/6865 (2018.01); C12Q 1/6867 (2018.01); C12Q 1/6869 (2018.01); C12Q 1/6874 (2018.01); C12Q 1/6806 (2018.01); B01L 7/00 (2006.01);
U.S. Cl.
CPC ...
C12Q 1/686 (2013.01); B01L 3/502707 (2013.01); B01L 3/502715 (2013.01); C07H 21/00 (2013.01); C12N 15/1075 (2013.01); C12N 15/1093 (2013.01); C12Q 1/6806 (2013.01); C12Q 1/6834 (2013.01); C12Q 1/6844 (2013.01); C12Q 1/6865 (2013.01); C12Q 1/6867 (2013.01); C12Q 1/6869 (2013.01); C12Q 1/6874 (2013.01); G01N 21/253 (2013.01); G01N 21/6428 (2013.01); G01N 21/6452 (2013.01); G01R 33/1269 (2013.01); B01L 3/5027 (2013.01); B01L 3/5085 (2013.01); B01L 7/52 (2013.01); B01L 2300/0636 (2013.01); B01L 2300/0819 (2013.01); B01L 2300/0877 (2013.01); G01N 21/6458 (2013.01); G01N 2021/6484 (2013.01); Y02P 20/582 (2015.11); Y10T 436/143333 (2015.01);
Abstract

Disclosed are methods for nucleic acid amplification wherein nucleic acid templates, beads, and amplification reaction solution are emulsified and the nucleic acid templates are amplified to provide clonal copies of the nucleic acid templates attached to the beads. Also disclosed are kits and apparatuses for performing the methods of the invention.


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