The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Mar. 27, 2007
Filed:
Apr. 30, 2003
Daria Jean Hazuda, Doylestown, PA (US);
Elizabeth Chen Dodson, Souderton, PA (US);
Ming-tain Lai, Lansdale, PA (US);
Min Xu, Ambler, PA (US);
Xiao-ping Shi, Warrington, PA (US);
Adam J. Simon, Langhorne, PA (US);
Guoxin Wu, Merion Station, PA (US);
Yueming LI, New York, NY (US);
Bruce Register, Berwyn, PA (US);
Daria Jean Hazuda, Doylestown, PA (US);
Elizabeth Chen Dodson, Souderton, PA (US);
Ming-Tain Lai, Lansdale, PA (US);
Min Xu, Ambler, PA (US);
Xiao-Ping Shi, Warrington, PA (US);
Adam J. Simon, Langhorne, PA (US);
Guoxin Wu, Merion Station, PA (US);
Yueming Li, New York, NY (US);
Bruce Register, Berwyn, PA (US);
Merk & Co., Inc., Rahway, NJ (US);
Abstract
Provided are methods of identifying inhibitors of β-secretase that employ modified β-secretase substrates. The modified β-secretase substrates have β-secretase cleavage sites that are altered from wild type. The amino acid sequences of the altered β-secretase cleavage sites contain different amino acids in at least one of the positions P2-P1-P1'-P2′ of the β-secretase cleavage site. Many of the modified β-secretase substrates are more efficient substrates for β-secretase than are corresponding substrates having wild-type sequences, that is, these modified substrates are more susceptible to enzymatic breakdown by β-secretase. Recombinant polynucleotide molecules encoding the modified β-secretase substrates are provided. Antibodies that recognize cleavage products of the modified β-secretase substrates are provided. Stable cell lines expressing the modified β-secretase substrates are provided. Transgenic animals expressing the modified β-secretase substrates are provided.